Level of expression of HGF after treatment with H
and/or HGF. Cells were serum-starved and treated with H2O2 (100 μM) and/or HGF (10 ng/ml). The level of HGF mRNA was measured by real-time RT-PCR analysis. Values are the means ± SD of triplicates of three independent experiments. Statistical significance was estimated by Student's t-test (*, p < 0.05;**, p < 0.01).