Figure 9

Effects of NAC on in vitro invasiveness. Cells in RPMI 1640 media supplemented with 5% FBS were placed in the upper chamber of Matrigel chamber and treated with or without NAC. The bottom chamber was filled with media containing 5% FBS and HGF with or without NAC. After 48 h of incubation, the cells which migrated through the filter were counted under light microscopy (10 fields at 200× power). Values are the means ± SD of triplicates of three independent experiments. Statistical significance was estimated by Student's t-test (*, P < 0.05; **, p < 0.01).