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Figure 4 | Journal of Experimental & Clinical Cancer Research

Figure 4

From: Upregulation of CENP-H in tongue cancer correlates with poor prognosis and progression

Figure 4

Knock down of CENP-H inhibits the proliferation of Tca8113 cells. (A) Effect of CENP-H knockdown in proliferation of Tca8113 was determined by MTT assays. (B) BrdU incorporation assay (upper panel) and colony formation assay (lower upper). Upper: The cells were fixed and subjected to BrdU staining and visualization under a fluorescence microscope. Data were obtained from three independent experiments with similar results. Green:Brdu; Blue:DAPI. Lower: The photographs of crystal violet stained Tca8113/control siRNA and Tca8113/CENP-H siRNA. Data were obtained form three independent experiments with similar results. (C) Cell lysates were prepared for western blot analysis of antibodies against CENP-H and Survivin. α-Tubulin was detected as an internal control.

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