Table 1 Primer Sequences Used for Reverse Transcription-PCR and Real-time Quantitative RT-PCR (5' to 3')
From: Upregulation of CENP-H in tongue cancer correlates with poor prognosis and progression
| Gene | Forward primer | Reverse primer | Probe | |
|---|---|---|---|---|
| RT-PCR | CENP-H | TGCAAGAAAAGCAAATCGAA | ATCCCAAGATTCCTGCTGTG | |
| GAPDH | CCACCCATGGCAAATTCCATGGCA | TCTAGACGGCAGGTCAGGTCCAC | ||
| Real-time PCR | CENP-H | CCTTATTTTGGGGAGTAAAGTCAAT | ACAAATGCACAGAAGTATTCCAAAT | FAM-TTCCTTAAGGGCAGGATCCT-TAMRA |
| GAPDH | GACTCATGACCACAGTCCATGC | AGAGGCAGGGATGATGTTCTG | FAM-CATCACTGCCACCCAGAAGACTGTG-TAMRA |