Anti-tumor efficacy of Ad-PEDF in vivo. Three groups of C57BL/6 mice bearing B16-F10 melanoma were treated with NS or 5 × 108 IU Ad-PEDF or Ad-Null at day 9, 12, 15, 18 and 21 after inoculation, respectively. Tumor sizes on each mouse were measured every 3 days and survival in each group was monitored daily. A. Significant differences were found in tumor volume (p < 0.05) between Ad-PEDF treated and the control groups. B. Significant increase of survival rate and prolonged survival times were observed in Ad-PEDF treated mice (log-rank test, *, p < 0.05, vs controls). n = 8.