Figure 5

TUNEL, CD31 and histological staining for tumor tissue. On day 24 following inoculation, tumor tissue from tumor-bearing mice treated with NS (a), Ad-Null (b), or Ad-PEDF (c) were sectioned and stained with FITC-dUTP, CD31 mAb or H&E. A. Apoptotic cells (green) were identified by TUNEL and examined under a fluorescence microscope (Original magnification, ×200). B. ANOVA analysis detected significant differences in the apoptotic index between Ad-PEDF group and control groups (p < 0.05). C. Micrographs show tumor tissue sections stained with anti-CD31 antibody (Original magnification, × 200) after NS, Ad-Null or Ad-PEDF treatment. D. Quantitative results for microvessel density (MVD) in tumor tissue. Ad-PEDF group shows a significant decrease of MVD compared to control groups (p < 0.05). E. Micrographs show tumor tissue sections stained with H&E. Decreased density of vessels and noticeable necrosis was observed in tumors from Ad-PEDF treated mice (c). In contrast, tumor cells grew well with less necrosis in NS (a) or Ad-Null group (b). (Original magnification, ×400). n = 2; 3 sections/mouse.