Table 2 Quantitative Real-time PCR primers
From: Down-regulated miR-9 and miR-433 in human gastric carcinoma
Gene name | Primer sequence | Anneal temperature(°C) | Product length (bp) |
|---|---|---|---|
U6 | F:5'GCTTCGGCAGCACATATACTAAAAT3' | 60 | 89 |
| Â | R:5'CGCTTCACGAATTTGCGTGTCAT3' | Â | Â |
hsa-miR-9 | F:5'GGGTCTTTGGTTATCTAGC3' | 60 | 64 |
| Â | R:5'TGCGTGTCGTGGAGTC3' | Â | Â |
hsa-miR-433 | F:5'GGATCATGATGGGCTCCT3' | 60 | 63 |
| Â | R:5'CAGTGCGTGTCGTGGAGT3' | Â | Â |