Phenotype-dependent apoptosis signalling in mesothelioma cells after selenite exposure

Table 2 Selenite-induced loss of mitochondrial membrane potential and effects of inhibition of apoptosis signalling enzymes

	Epithelioid cells			Sarcomatoid cells
Inhibitor	Loss of δΦ _m after selenite treatment ^a	Statistical significance vs. no selenite ^b	Statistical significance vs. selenite only ^c	Loss of δΦ _m after selenite treatment ^a	Statistical significance vs. no selenite ^b	Statistical significance vs. selenite only ^c
Positive control	2.89 (± 0.68)			1.28 (± 0.18)
Selenite	3.41 (± 0.57)	p < 0.01		3.30 (± 0.24)	p < 0.001
JNK	0,94 (± 0.06)			1.05 (± 0.05)
JNK + selenite	3,96 (± 0.58)	p < 0.001	ns	3.74 (± 0.25)	p < 0.001	ns
p38	0.99 (± 0.04)			0.88 (± 0.03)
p38 + selenite	4.06 (± 0.63)	p < 0.001	ns	4.15 (± 0.52)	p < 0.001	ns
p53	0.74 (± 0.05)			0.92 (± 0.03)
p53 + selenite	2.62 (± 0.57)	p < 0.05	ns	3.59 (± 0.52)	p < 0.001	ns
Cathepsin B	1.27 (± 0.12)			1.46 (± 0.10)
Cathepsin B + selenite	5.68 (± 0.70)	p < 0.001	ns	6.27 (± 0.75)	p < 0.001	p < 0.01
Cathepsin D, E	0.93 (± 0.06)			0.90 (± 0.03)
Cathepsin D, E + selenite	3.95 (± 0.77)	p < 0.001	ns	3.45 (± 0.37)	p < 0.001	ns

a: Fold change in JC-1 green fluorescence. Range shows the standard error of the mean (SEM).
b: One-way ANOVA analyses were performed with Bonferroni's multiple comparisons test.
c: One-way ANOVA analyses were performed with Dunnett's post test. ns = not significant.

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