Figure 2

SIAH-1 protein expression in normal and tumor tissues. Normal breast (a), liver (b) and lung (c) normal tissues and its respective tumor counterpart from the same patient (d), (e) and (f) are showed. Paraffined tissues were stained with anti-SIAH-1 antibody, and detected with a secondary antibody conjugated to Rhodamine Red-X. Cells were counterstained with DioC6 (green) to mark the ER, cellular membranes, and mitochondria.