Table 1 Primers and TaqMan probes used to quantify mRNA expression of SIAH-1, Kid/KIF22 and β 2 microglobulin.
Gen | PCR Product (bp) | Primers | TaqMan Probe |
|---|---|---|---|
SIAH-1 | 75 | 5'-GCTAAATGGTCATAGGCGACG-3' 5'-ATGGCTGTTGCAATTCCTTCAT-3' | 5'-TTGACTTGGGAAGCGACTCCTCGATCTA-3' |
Kid/KIF22 | 76 | 5'-CGGCCTTTTACCAATGAGAGC-3' 5'-GACCAAGCAATTCTTTCTGAGACA-3' | 5'-CAGCCTCATGCCTTGGGACCTGTTAAG-3' |
β 2 microglobulin | 67 | 5'-CGCTCCGTGGCCTTAGC-3' 5'-GAGTACGCTGGATAGCCTCCA-3' | 5'-TGCTCGCGCTACTCTCTCTTTCTG-3' |