Figure 3

Western blotting analysis of gene expression. Stably TGF-β1-transfected BxPC3 cells were grown and treated with G418, and total cellular protein was isolated and subjected to Western blotting analysis. α-SMA, a mesenchymal marker, is responsible for the enhanced cell mobility. CyclinD1 is responsible for cell growth, while p21WAF1 is involved in cell growth arrest.