Figure 3

Reduction of transformed phenotype by knockdown of CBX7 expression. A) CBX7 knockdown in SGC-7901 cells resulted in the upregulation of p16 as determined by Western blot analysis. β-actin was used as a loading control. The level of p16 was quantified by densitometric analysis of signal present in each lane and normalizing it to β-actin signal of respective lane using ImageJ 1.37v software (NIH, Bethesda, USA). B) Knockdown of CBX7 expression in SGC-7901 cells resulted in increased cellular senescence (p < 0.01; upper panel, pictures of SA-β-gal stained cells; lower panel, senescent cells were counted and plotted). C) Decreased number of colonies in soft agar in CBX7 knockdown cells (p < 0.01; upper panel, pictures of colonies in soft agar; lower panel, the number of colony were counted and plotted). D) Transwell migration assays using the Corning chamber showed that fewer number of cells migrated in SGC-7901 cells with CBX7 knockdown compared with that in control (p < 0.01; upper panel, pictures of migrated cells; lower panel, the number of migrated cells were counted and plotted).