Figure 1
A: Schematic diagram of the anti-CD20scFvFc-pLNCX and anti-CD20scFvFc/CD28/CD3ζ pLNCX, LTR: long term repeat, Neo: neomycin, CMV: cytomegalovirus. B: The CD3, CD4 and CD8 antigens on surface of PBMCs, which incubated for 10 days after stimulation by PHA-L, OKT3 and IL-2 were analyzed by flow cytometry. A life gate was set around CD3 positive cells; only those cells expressing this membrane protein were included, and 20,000 events were analyzed. C: PBMCs grafted with anti-CD20scFvFc/CD28/CD3ζ after selected by G418 for 7 days and analysis of PBMCs grafted with anti-CD20scFvFc/CD28/CD3ζ by Western blot. D-a: PBMCs grafted with anti-CD20scFvFc/CD28/CD3ζ co-culture with Raji cells for 12 hours. D-b: PBMCs grafted with anti-CD20scFvFc/CD28/CD3ζ co-culture with Raji cells for 24 hours. E: Cell lysis evaluated by [3H]TdR release assay. (In experimental group, *represents p < 0.05 compared to control group at the same time point).