Table 1 The sequences of the primers used in the experiment
Gene | Sense | Antisense | Product (bps) |
|---|---|---|---|
HIF1α | TGCACAGGCCACATTCACGT | GTTCACAAATCAGCACCAAGC | 97 |
Flk-1 | ACAGTGGTATGGTTCTTGCCTCA | GTAGCCGCTTGTCTGGTTTGA | 140 |
VEGF | TCACCAAGGCCAGCACATAG | GGGAACGCTCCAGGACTTAT | 166 |
Cyclin D1 | GATGCCAACCTCCTCAACGAC | CTCCTCGCACTTCTGTTCCTC | 171 |
V-src | CACTCGCTCAGCACAGGACAG | AGAGGCAGTAGGCACCTTTCG | 196 |
P53 | GCTGCTCAGATAGCGATGGTC | CTCCCAGGACAGGCACAAACA | 298 |
β-actin | CCTGTACGCCAACACAGTGC | ATACTCCTGCTTGCTGATCC | 211 |