Chromogenic in situ hybridization to detect EGFR gene copy number in cell blocks from fine-needle aspirates of non small cell lung carcinomas and lung metastases from colo-rectal cancer

Table 2 Comparison between immunohistochemistry, CISH and FISH in 33 cell blocks from lung FNAC

IHC score	N° of cases	CISH				FISH
		D	T	P	A	D	T	P	A
0	9	1	5	3	0	2	2	5	0
1+	3	1	0	2	0	0	0	3	0
2+	5	1	0	4	0	0	0	5	0
3+	16	2	7	6	2	2	5	6	3
Total	33	5	12	14	2	4	7	19	3

IHC: immunohistochemistry; CISH: chromogenic in situ hybridization; FISH: fluorescence in situ hybridization;
D: disomy, 1.6-2.0 balanced gene and chromosome 7; T: trisomy, 2.2-3.0 balanced gene and chromosome 7; P: polysomy, 3.1-4.4 balanced gene and chromosome 7; A: amplified, gene-to-chromosome 7 ratio ≥2
Sensitivity 60%; Specificity 89%; Positive predictive value 50%; Negative predictive value 93%

ISSN: 1756-9966