Skip to main content

Table 2 Comparison between immunohistochemistry, CISH and FISH in 33 cell blocks from lung FNAC

From: Chromogenic in situ hybridization to detect EGFR gene copy number in cell blocks from fine-needle aspirates of non small cell lung carcinomas and lung metastases from colo-rectal cancer

IHC score N° of cases CISH FISH
   D T P A D T P A
0 9 1 5 3 0 2 2 5 0
1+ 3 1 0 2 0 0 0 3 0
2+ 5 1 0 4 0 0 0 5 0
3+ 16 2 7 6 2 2 5 6 3
Total 33 5 12 14 2 4 7 19 3
  1. IHC: immunohistochemistry; CISH: chromogenic in situ hybridization; FISH: fluorescence in situ hybridization;
  2. D: disomy, 1.6-2.0 balanced gene and chromosome 7; T: trisomy, 2.2-3.0 balanced gene and chromosome 7; P: polysomy, 3.1-4.4 balanced gene and chromosome 7; A: amplified, gene-to-chromosome 7 ratio ≥2
  3. Sensitivity 60%; Specificity 89%; Positive predictive value 50%; Negative predictive value 93%