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Table 3 Comparison between CISH and FISH in 33 cell blocks from lung FNAC

From: Chromogenic in situ hybridization to detect EGFR gene copy number in cell blocks from fine-needle aspirates of non small cell lung carcinomas and lung metastases from colo-rectal cancer

CISH FISH
EGFR status Chromosome 7 EGFR status Chromosome 7
  Disomy Polysomy   Disomy Polysomy
31 NA 6 (19%) 25 (81%) 30 NA 4 (13%) 26 (87%)
  Disomy Polysomy   Disomy Polysomy
2 A 0 2 (100%) 3 A 1 (33%) 2 (67%)
Total 33 6 27 33 5 28
  1. CISH: chromogenic in situ hybridization; FISH: fluorescence in situ hybridization; NA: not amplified, gene-to-chromosome 7 ratio < 2; A: amplified, gene-to-chromosome 7 ratio ≥2; Disomy: 1.6-2.0 balanced gene and chromosome 7; Polysomy: 3.1-4.4 balanced gene and chromosome 7
  2. Concordance 97%; k = 0.78; p < 0.0001; Sensitivity 67%; Specificity 100%; Positive predictive value 100%; Negative predictive value 97%