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Figure 4 | Journal of Experimental & Clinical Cancer Research

Figure 4

From: Reduction of lung metastasis, cell invasion, and adhesion in mouse melanoma by statin-induced blockade of the Rho/Rho-associated coiled-coil-containing protein kinase pathway

Figure 4

Statins specifically suppress the Rho/ROCK pathway. (A) B16BL6 cells were treated with 0.05 μM fluvastatin or 0.1 μM simvastatin for 3 d. Rho expression was determined by immunoblotting analysis of the membrane and cytoplasmic fractions by using the anti-Rho antibody. The expression of phosphorylated LIMK and MLC was determined by immunoblotting analysis of the whole-cell lysate using phosphorylated LIMK (phospho-LIMK) and phosphorylated MLC (phospho-MLC). (B) B16BL6 cells, which had been treated with 75 μM Y27632 for 3 d, were injected into the tail veins of syngeneic C57BL/6J mice. After 14 d, visible nodules that metastasized to the lung were counted. The results are expressed as the means ± S.D. of 9 mice. (C) B16BL6 cells were treated with 75 μM Y27632 for 3 d. The expression of phosphorylated LIMK and MLC was determined by immunoblotting analysis of the whole-cell lysate using phosphorylated LIMK (phospho-LIMK), phosphorylated MLC (phospho-MLC), and β-actin (internal standard).

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