Figure 2

Mutation detection success on varying the amount of input DNA. The DNA yield was grouped into categories and the percentage of mutations detected calculated for each group. The n values are the successful number of sequencing and ARMS analyses. The lower yielding samples did not show any decrease in the numbers of BRAF or NRAS mutations detected. Both DNA sequencing and ARMS gave similar results in each category although overall ARMS detected more mutations. As the DNA concentration increased the number of successful sequencing reactions also increased: at >50 copies per assay input, the analysis success rate was very similar for both ARMS and sequencing.