Figure 5

Confocal immunofluorescence of fibronectin expression in mesothelial cells. Serum-starved HPMCs were incubated with TGF-β1 for up to 72 h, and fixed for immunostaining with a polyclonal antibody against fibronectin. Fibronectin was visualized by FITC (green), and nuclei were visualized by To-PRO-3 (blue) under immunofluorescence confocal microscopy. A, Control cells. B, Mesothelial cells treated with TGF-β1 (5 ng/ml) for 72 h. All photos were obtained at 100× magnification.