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Figure 7 | Journal of Experimental & Clinical Cancer Research

Figure 7

From: Induction of gastric cancer cell adhesion through transforming growth factor-beta1-mediated peritoneal fibrosis

Figure 7

Effects of TGF-β1 and RGD on adhesion of gastric cancer cells to mesothelial cells. A, Gastric cancer HGC-27 and HSC-39 cells were grown and treated with or without TGF-β1 (5, 10, 20 ng/ml) for 48 h and then subjected to cell adhesion assay. B, HPMCs were incubated with TGF-β1 and HGC-27 cancer cells were pretreated with or without RGD, and then cancer cells were added onto the mesothelial cell culture and subjected to cell adhesion assay. C, HPMCs were incubated with TGF-β1 and HSC-39 cancer cells were pretreated with or without RGD, and then cancer cells were added onto the mesothelial cell culture and subjected to cell adhesion assay. D, Fluorescence microscopy (x 40) of gastric cancer HGC-27 cells adhered to the confluent mesothelial cells. a, mesothelial cells without TGF-β1 treatment; b, mesothelial cells treated with 5 ng/ml TGF-β1 for 48 h; c, gastric cancer HGC-27 cells were pretreated with RGD, and then added onto the mesothelial cells that were pretreated with TGF-β1 (5 ng/ml) for 48 h. * p < 0.05 as compared with control.

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