Figure 4
From: Estrogen receptor α (ERα) mediates 17β-estradiol (E2)-activated expression of HBO1
E2 enhances HBO1 expression through ERK1/2 signaling pathways. (A) Serum-starved T47 D cells were treated with E2 (10-8 M), or U0126 (10 uM) plus E2 (10-8 M) for 24 hours. Then equal amounts of protein (lysates) were subjected to SDS-PAGE. Western blot was performed using the Anti-phospho-ERK1/2 (Thr202/Tyr204), anti-HBO1 and anti-ERK1/2 antibodies. GAPDH was used as an internal control. (B) Serum-starved MCF-7 cells were treated with E2 (10-8 M), or U0126 (10 uM) plus E2 (10-8 M) for 24 hours. Western blot was performed as described in (A).