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Table 3 Cycle threshold (Ct) values of candidate endogenous control genes across all tissue samples.

From: Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer

Gene Mean ± s.e.m Standard deviation (SD) Ct min Ct max Ct Range CtCV%
18S 8.708 0.151 1.314 6.858 16.932 10.073 14,99
ACTB 20.236 0.187 1.630 17.979 31.018 13.039 8,10
B2M 17.101 0.150 1.306 15.251 23.587 8.336 7,69
GAPDH 19.358 0.191 1.661 17.382 30.403 17.382 8,63
GUSB 23.748 0.150 1.309 21.719 32.338 10.619 5,55
HMBS 26.239 0.186 1.625 24.315 36.823 12.508 6,23
HPRT1 23.649 0.164 1.429 21.852 31.617 9.765 6,04
IPO8 25.085 0.146 1.276 23.749 33.903 10.154 5,12
PGK1 21.025 0.285 2.487 8.354 29.829 21.474 10,35
POLR2A 24.717 0.184 1.606 20.827 34.874 14.047 6,54
PPIA 17.978 0.150 1.305 15.980 25.150 9.170 7,34
RPLP0 20.452 0.162 1.413 18.682 29.171 10.489 6,93
TBP 26.704 0.194 1.692 24.858 38.656 13.799 6,38
TFRC 22.878 0.196 1.711 19.907 32.261 12.354 7,53
UBC 21.665 0.163 1.422 19.475 30.387 10.912 6,60
YWHAZ 24.720 0.193 1.685 22.733 32.853 10.120 6,86
  1. Note. S.e.m, standard error of mean; CtCV%, Coefficients of variations of candidate reference genes.