Skip to main content

Table 3 Cycle threshold (Ct) values of candidate endogenous control genes across all tissue samples.

From: Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer

Gene

Mean

± s.e.m

Standard deviation (SD)

Ct min

Ct max

Ct Range

CtCV%

18S

8.708

0.151

1.314

6.858

16.932

10.073

14,99

ACTB

20.236

0.187

1.630

17.979

31.018

13.039

8,10

B2M

17.101

0.150

1.306

15.251

23.587

8.336

7,69

GAPDH

19.358

0.191

1.661

17.382

30.403

17.382

8,63

GUSB

23.748

0.150

1.309

21.719

32.338

10.619

5,55

HMBS

26.239

0.186

1.625

24.315

36.823

12.508

6,23

HPRT1

23.649

0.164

1.429

21.852

31.617

9.765

6,04

IPO8

25.085

0.146

1.276

23.749

33.903

10.154

5,12

PGK1

21.025

0.285

2.487

8.354

29.829

21.474

10,35

POLR2A

24.717

0.184

1.606

20.827

34.874

14.047

6,54

PPIA

17.978

0.150

1.305

15.980

25.150

9.170

7,34

RPLP0

20.452

0.162

1.413

18.682

29.171

10.489

6,93

TBP

26.704

0.194

1.692

24.858

38.656

13.799

6,38

TFRC

22.878

0.196

1.711

19.907

32.261

12.354

7,53

UBC

21.665

0.163

1.422

19.475

30.387

10.912

6,60

YWHAZ

24.720

0.193

1.685

22.733

32.853

10.120

6,86

  1. Note. S.e.m, standard error of mean; CtCV%, Coefficients of variations of candidate reference genes.