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Table 4 Ranking and best combination of reference genes determined by geNorm, NormFinder and CtCV%.

From: Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer

Rank

GeNorm

NormFinder

CtCV%

1

HPRT1

RPLP0

IPO8 (5.12)

2

PPIA

TBP

GUSB (5.55)

3

PGK1

GUSB

HPRT1 (6.04)

4

RPLP0

POLR2A

HMBS (6.23)

5

HMBS

IPO8

TBP (6.38)

6

GAPDH

GAPDH

POLR2A (6.54)

7

GUSB

PPIA

UBC (6.60)

8

IPO8

HPRT1

YWHAZ (6.86)

Best gene/combination

HPRT1/PP1A

IPO8/PPIA

IPO8