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Table 4 Ranking and best combination of reference genes determined by geNorm, NormFinder and CtCV%.

From: Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer

Rank GeNorm NormFinder CtCV%
1 HPRT1 RPLP0 IPO8 (5.12)
2 PPIA TBP GUSB (5.55)
3 PGK1 GUSB HPRT1 (6.04)
4 RPLP0 POLR2A HMBS (6.23)
5 HMBS IPO8 TBP (6.38)
6 GAPDH GAPDH POLR2A (6.54)
7 GUSB PPIA UBC (6.60)
8 IPO8 HPRT1 YWHAZ (6.86)
Best gene/combination HPRT1/PP1A IPO8/PPIA IPO8