Figure 4
From: Involvement of mitochondrial pathway in NCTD-induced cytotoxicity in human hepG2 cells
Effect of NCTD on ROS generation in HepG2 cells. (A) Cells were treated with NCTD for 6 h, followed by staining with DCHF-DA (100 μM) for an additional 30 min. NAC(10 mM) was added 1 h prior to the treatment with 20 μg/ml NCTD for 6 h.Cells treated with NCTD showed a dose-dependent increase in ROS generation. The horizontal axis represents DCFH-DA fluorescence and the vertical axis represents cell count. (B) *P < 0.01 vs Control, §P < 0.05 vs 10 μg/ml NCTD, ▲P < 0.05 vs 20 μg/ml NCTD, #P < 0.01 vs 20 μg/ml NCTD