Expression of miR-145 in normal tissues and non-small cell lung cancer. miR-145 levels were measured by miRNA TaqMan qRT-PCR in normal and in NSCLC tissue (A), and in the normal lung cell line Gekko Lung-1, and the NSCLC cell lines A549 and H23 (B). (A) Relative levels of miR-145 were lower in tumor tissue than in normal tissue. (B) Relative levels of miR-145 in the NSCLC cell lines, particularly A549, were lower than in Gekko Lung-1 cells. Vertical axis indicates relative expression of each miRNA normalized to control. Results are mean ± SD of three independent experiments. *P < 0.05 by Student's paired t-test compared to untreated cells (control).