Figure 4

miR-145 regulates the c-myc/eIF4E pathway in NSCLCs. Western blot analysis of c-myc, eIF4E, and CDK4 expression levels in normal and tumor tissue (A, B), and one normal lung cell line and two NSCLC cell lines (C, D). (E, F) Western blot for c-myc, eIF4E, and CDK4 after transfection with pre-miR-145 expression vector and or control miRNA vector. (G) Cells transiently transfected with the empty pBV-luc plasmid vector or pBV-c-Myc-luc plasmid were treated for 24 h. Luciferase activity was normalized to protein concentration and then to measurements from pBV-luc-transfected, DMSO-treated control cultures. (H) ChIP assays of c-Myc binding to miR-145 DNA. The beta actin gene was used as an internal control.