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Table 2 Reactivity of FU-MFH-2 cells, in vitro and in vivo, including the original tumor cells with various antibodies.

From: Establishment of a new human pleomorphic malignant fibrous histiocytoma cell line, FU-MFH-2: molecular cytogenetic characterization by multicolor fluorescence in situ hybridization and comparative genomic hybridization

Antibody

FU-MFH-2 cells

Original tumor cells

 

in vitro

in vivo

 

Vimentin

+ + +

+ + +

+ + +

EMA

-

-

-

AE1/AE3

-

-

-

CAM 5.2

-

-

-

Desmin

-

-

-

α-SMA

-

-

-

MSA

-

-

-

S-100 protein

-

-

-

NSE

-

-

-

CD68

+ + +

+ +

+ +

Lysozyme

-

-

+

AAT

-

-

-

ACT

-

-

-

C-Kit

-

-

-

  1. Abbreviations: EMA, epithelial membrane antigen; α-SMA, alpha-smooth muscle actin; MSA, muscle-specific actin; NSE, neuron-specific enolase; AAT, alpha-1-antitrypsin; ACT, alpha-1-antichymotrypsin.
  2. + + +, > 75% positive cells; + +, 15-75% positive cells; +, < 15% positive cells, -, negative reaction.