Figure 1

Immunofluorescence staining of GSK-3β in ALL cells. Bone marrow samples were obtained from children with ALL and from control patients. GSK-3β was probed with Dylight 549-labeled anti-rabbit secondary antibody (red fluorescence) and nuclei were counterstained with Hoechst 33342 (blue fluorescence). Nuclear accumulation of GSK-3β in ALL cells was detected, whereas only cytoplasmic expression of GSK-3β was observed in control cells.