Figure 1
From: Expression of MDR1, HIF-1α and MRP1 in sacral chordoma and chordoma cell line CM-319
Immunohistochemical staining of HIF-1α, MDR1 and MRP1 in chordoma, CM-319 and nucleus pulpous. With immunohistochemical staining, the expression of chemotherapy resistant proteins using primary antibody to HIF-1α (A, B, G), MDR1 (E, F, I) and MRP1 (C, D, H) was determined in chordoma (B, D, F) and CM-319 (A, C, E). Intense membrane and cytoplasmic staining of MRP1 (×400) and cytoplasmic and nuclus staining of HIF (×400). Negative immunostaining of MDR1 was found in chordoma and CM-319. In control, negative immunostaining of HIF-1, MRP1 and MDR1 (G, H, I) was found in nucleus pulposus.