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Figure 4 | Journal of Experimental & Clinical Cancer Research

Figure 4

From: Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells

Figure 4

ROS accumulation contributes to the synergistic cytotoxicity induced by saikosaponins plus cisplatin in HeLa cells. (A) and (B) HeLa cells were pretreated with BHA (100 μM) or NAC (1 mM) for 30 min or remained untreated and then treated with saikosaponin-a (10 μM) or saikosaponin-d (2 μM) or cisplatin (8 μM) individually or combination of saikosaponin and cisplatin for 48 h. Cell death was measured as described in Fig. 1A. (C) and (D) HeLa cells were pretreated with NAC (1 mM) for 30 min or remained untreated and then treated with saikosaponin-a (10 μM) or saikosaponin-d (2 μM) or cisplatin (8 μM) alone or combination of saikosaponin and cisplatin for another 30 min. Cells were stained with DHE (C) or CM-H2DCFDA (D) 30 min before collecting cells and then analyzed by flow cytometer.

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