Figure 1From: Antiproliferative factor decreases Akt phosphorylation and alters gene expression via CKAP4 in T24 bladder carcinoma cellsCKAP4 knockdown in T24 cells. A, Western blot analysis of CKAP4 protein expression in cells electroporated in the presence of no siRNA (Lanes 1 and 2), CKAP4 siRNA (Lanes 3 and 4), or scrambled non-target (NT) siRNA (Lanes 5 and 6), and treated with as-APF (APF) or its inactive control peptide (Pep). β-actin served as a standard control. B, Inhibition of 3H-thymidine incorporation by as-APF (APF) in cells electroporated with no siRNA, CKAP4 siRNA, or non-target siRNA. Results are shown as percent inhibition of 3H-thymidine incorporation compared to control cells that did not receive as-APF treatment. Experiment was performed in triplicate twice.Back to article page