CKAP4 knockdown in T24 cells. A, Western blot analysis of CKAP4 protein expression in cells electroporated in the presence of no siRNA (Lanes 1 and 2), CKAP4 siRNA (Lanes 3 and 4), or scrambled non-target (NT) siRNA (Lanes 5 and 6), and treated with as-APF (APF) or its inactive control peptide (Pep). β-actin served as a standard control. B, Inhibition of 3H-thymidine incorporation by as-APF (APF) in cells electroporated with no siRNA, CKAP4 siRNA, or non-target siRNA. Results are shown as percent inhibition of 3H-thymidine incorporation compared to control cells that did not receive as-APF treatment. Experiment was performed in triplicate twice.