Figure 3From: Antiproliferative factor decreases Akt phosphorylation and alters gene expression via CKAP4 in T24 bladder carcinoma cellsAkt phosphorylation activity in T24 bladder cancer cells. A, Western blot analysis of Akt protein expression and phosphorylation in cells electroporated in the presence of no siRNA (Lanes 1 and 2), CKAP4 siRNA (Lanes 3 and 4), or scrambled non-target (NT) siRNA (Lanes 5 and 6), and treated with as-APF (APF) or its inactive control peptide (Pep). β-actin served as a standard control. B, Quantitative real time RT-PCR analysis of Akt mRNA expression in T24 cells electroporated with no siRNA, C, CKAP4 siRNA, or D, non-target siRNA, and then treated with as-APF (APF) or its inactive control peptide (Pep). Each experiment was performed in duplicate on at least three separate occasions. Data are expressed as mean ± SEM.Back to article page