GSK3β tyr216 phosphorylation activity in bladder cancer cells. A, Western blot analysis of GSK3β protein expression and phosphorylation in cells electroporated in the presence of no siRNA (Lanes 1 and 2), CKAP4 siRNA (Lanes 3 and 4), or scrambled non-target (NT) siRNA (Lanes 5 and 6), and treated with as-APF (APF) or its inactive control peptide (Pep). β-actin served as a standard control. B, Quantitative real time RT-PCR analysis of GSK3β mRNA expression in T24 cells electroporated with no siRNA, C, CKAP4 siRNA, or D, non-target siRNA, and then treated with as-APF (APF) or its inactive control peptide (Pep). Each experiment was performed in duplicate on at least three separate occasions. Data are expressed as mean ± SEM.