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Table 1 Reverse transcription and stem-loop primers for real-time RT-PCR

From: MicroRNAs involved in neoplastic transformation of liver cancer stem cells

Gene name

Reverse transcription primer (5'-3')

PCR primers (5'-3')

F: forward primer

R: reverse primer

miR-21

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCAACA

F: CGCGCTAGCTTATCAGACTGA

  

R: GTGCAGGGTCCGAGGT

miR-10b

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCACAAA

F: CGTCGTACCCTGTAGAACCGA

R: GTGCAGGGTCCGAGGT

miR-470*

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCTTCT

F: GTGCGAACCAGTACCTTTCTG

R: GTGCAGGGTCCGAGGT

miR-34c-3p

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCTGGC

F:GGTGGAATCACTAACCACACG

R: GTGCAGGGTCCGAGGT

let-7i*

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGCAAG

F: TAGTACTGCGCAAGCTACTGC

R: GTGCAGGGTCCGAGGT

miR-200a*

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCCAGC

F: GAGTGCATCTTACCGGACAGT

R: GTGCAGGGTCCGAGGT

miR-148b*

GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGCCTGA

F: GGCGCAAGTTCTGTTATACAC

R: GTGCAGGGTCCGAGGT

U6

CGCTTCACGAATTTGCGTGTCAT

F: GCTTCGGCAGCACATATACTAAAAT

R: CGCTTCACGAATTTGCGTGTCAT