Figure 1From: Transcription factor c-Myb promotes the invasion of hepatocellular carcinoma cells via increasing osteopontin expressionVerification of difference of OPN and c-Myb expression in HCC cell lines. HCCLM6 cells expressed high level of OPN and c-Myb compared with SMMC-7721 cells. (A) Relative OPN and c-Myb mRNA levels in different cell lines by RT-PCR analysis. (B) Real-time quantitative PCR analysis confirmed the difference of c-Myb mRNA expression in different cell lines. Graph depicted relative expression of OPN mRNA normalized to that of GAPDH. The mRNA expression of c-Myb in HCCLM6 was used as control. Data expressed as means ± SD (*P < 0.05, SMMC-7721 vs. HCCLM6). (C)Western blot analysis of OPN and c-Myb protein expression in HCC cell line SMMC-7721 and HCCLM6. Blot was representative of three experiments.Back to article page