Skip to main content
Figure 2 | Journal of Experimental & Clinical Cancer Research

Figure 2

From: Transcription factor c-Myb promotes the invasion of hepatocellular carcinoma cells via increasing osteopontin expression

Figure 2

Electrophoretic mobility shift sssays (EMSA) of c-Myb binding to OPN promoter and transient transfection analysis of OPN promoter activity. (A). EMSA were performed using nuclear extract prepared from SMMC-7721 and HCCLM6 cells. Assays utilized a labeled probe of 25-nt fragment containing the area of c-Myb binding site in the OPN promoter or a mutant form of the c-Myb binding site (c-Myb-binding site TAACGG was mutated to TAT CGG). The blot was representative of three experiments. (B) To confirm the role of c-Myb in the increased OPN protein expression in HCCLM6 cells, Human OPN promoter (-1488 to +185 nt) was cloned into the pGL3-basic luciferase reporter vector. The OPN promoter reporter constructs were transfected into HCCLM6 cells. In certain instances, c-Myb siRNA or scramble siRNA was co-transfected. Luciferase activity was normalized to that of β-galactosidase activity. Data are presented as means ± SD of three experiments. (*P < 0.05, c-Mb siRNA-treated group vs. scramble siRNA group).

Back to article page