Comparative analysis of cell death induction by Taurolidine in different malignant human cancer cell lines

Table 1 Effect of increasing Taurolidine concentrations on viable, apoptotic and necrotic cells in different cell lines.

	HT29	Chang Liver	HT1080	AsPC-1	BxPC-3
FACS analysis
Reduction of viable cells after 6 h	TRD 250	TRD 1000	TRD 1000 TRD 100	TRD 1000	TRD 1000 TRD 250
Increase of apoptotic cells after 6 h	TRD 250	TRD 1000 TRD 250	TRD 1000 TRD 100	TRD 1000	TRD 1000 TRD 250
Increase of necrotic cells after 6 h	Ø	TRD 1000	TRD 1000	TRD 1000	TRD 1000
Reduction of viable cells after 24 h	TRD 250 TRD 1000	TRD 250 TRD 100 TRD 1000	TRD 100 TRD 250 TRD 1000	TRD 1000	TRD 1000 TRD 250 TRD 100
Increase of apoptotic cells after 24 h	TRD 250 TRD 1000	TRD 250 TRD 100 TRD 1000	TRD 100 TRD 250 TRD 1000	Ø	TRD 250
Increase of necrotic cells after 24 h	TRD 1000	TRD 250 TRD 100 TRD 1000	TRD 250 TRD 100 TRD 1000	TRD 1000	TRD 1000 TRD 250
Pattern of dose response (viable cells) after 24 h (FACS anaylsis)	V-shaped	V-Shaped	Anti-Prop.	Prop.	Prop.

Effect of increasing Taurolidin (TRD) concentrations (100 μM, 250 μM and 1000 μM) in different cell lines measured by FACS analysis (Annexin V/Propidium Iodide). TRD concentrations in μM with significant differences in viable, apoptotic or necrotic cells compared to untreated controls.
TRD = Taurolidin, Prop. = proportional, Anti-Prop. = anti-proportional
Ø = no significant effect
Bold print = TRD concentration (in μM) with the highest reduction of viable cells after 6 h and 24 h.

ISSN: 1756-9966