Figure 4
Effect of the MAPK inhibitors on the expression of VEGF-A mRNA. PC-14 cells were treated with 10 μM of each inhibitor for MEK (U0126), p38 (SB 202190), and JNK (JNK Inhibitor II), and specific mRNA was quantified 0, 6, 12, 24, and 48 h after treatment by using real-time PCR. Data were normalized relative to the level of 18S rRNA and expressed as mean (SD) (n = 3). *P < 0.05, ***P < 0.001 vs. vehicle control.