Figure 1

ChA21 inhibits the growth of SK-OV-3 cells in vitro and in vivo. (A) Cells were exposed to 0.067-5.4 μg/ml ChA21 for 72 h. (B) Cells were treated with ChA21 at the concentration of 5.4 μg/ml for 24, 48, 72, 96 h, respectively. OD 570 nm was measured by a multi-well scanning spectrophotometer. Significant differences are represented by asterisk (P < 0.05) and double asterisk (P < 0.01). (C, D) Female BALB/c nude mice were subcutaneously inoculated with human ovarian cancer cells SK-OV-3 (5 × 10⁶) into the left flank of mice. The mice were randomized and injeceted twice weekly via caudal vein with either sterile normal saline or ChA21 (40 mg/kg) for 5 weeks. Tumor size was measured twice a week and converted to tumor volume. ChA21 treatment group have a significantly reduced tumor volume compared with the controls (P < 0.05). Results are representative of the mean ± s.e.m. of 8 animals in each group.