Figure 2
From: Expression and aberrant promoter methylation of Wnt inhibitory factor-1 in human astrocytomas
Selected results of mRNA expression and promoter methylation of WIF-1. A, RT-PCR results of the WIF-1 gene in normal brain tissue (N1-N2) and astrocytoma (T1-T8) is shown. GAPDH is shown as a control. The fragments of amplified human WIF-1 and GAPDH cDNA are188 and135 bp, respectively. B, Representative methylation status of the WIF-1 promoter in 10 matched pairs of normal brain tissue (N1-N2) and astrocytomas(T1-T8).T1,3,5:WHO grade II;T2,4,6:WHO grade III;T7,8: WHO grade IV. U, unmethylated control;M, methylated control;NTC, no template control.