Figure 1

Detection of SPARC gene TRR methylation. (A) Illustration of the SPARC gene TRR and topology of the BSP primer. The black bar indicates the analyzed region. The bold "G" indicates the transcriptional start site. The bold italic "CG" indicates the location of 12 CpG island sites. The underlined sequence indicates the primers for BSP. Blue and red rectangles indicate the Sp1 and AP1 binding consensus sequences, respectively. The red triangles indicate the region whose representative sequence analyses were showed in Figure 1B. (B) Representative sequencing data of the SPARC gene TRR in four different groups of pancreatic tissues obtained using BSP PCR-based sequencing analysis. CpG dinucleotides "C" in the objective sequence are shown in red. The red, yellow, green, light blue, and deep blue dots under the analyzed sequence represent different methylation ratios, respectively.