Table 1 PCR primer used in the experiment
Target mRNA | Primer sequence5'-3' | Product Size (bp) | GenBank Accession No |
|---|---|---|---|
TβRII Sense | gca cgt tca gaa gtc ggt ta | 493 | D50683 |
Antisense | gcg gta gca gta gaa gat ga | ||
Smad2 Sense | aag aag tca gct ggt ggg t | 246 | AF027964 |
Antisense | gcc tgt tgt atc cca ctg a | ||
Smad3 Sense | cag aac gtc aac acc aagt | 308 | NM005902 |
Antisense | atg gaa tgg ctg tag tcg t | ||
Smad4 Sense | cca gga tca gta ggt gga at | 243 | U44378 |
Antisense | gtc taa agg ttg tgg gtc tg | ||
Smad7 Sense | gcc ctc tct gga tat ctt ct | 320 | AF015261 |
Antisense | gct gca taa act cgt ggt ca | ||
β-actin Sense | aca atg tgg ccg agg ctt t | 260 | M10277 |
Antisense | gca cga agg ctc atc att ca |