Assay for tumor-specific, CTL activity and IFN-γ secretion in immunized mice. (A), Splenic T cells from immunized mice were restimulated ex vivo by culturing with MMC-treated, MFC tumor cells. The restimulated T cells (effector cells) were incubated with target MFC or B16F10 cells for 20 h. Cytolytic activity (lysis) was determined. (B), Supernatants were collected for IFN-γ assay. All data are shown as means ± SD for 10 mice per group and are representative of three independent experiments. * P < 0.05.