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Table 2 Real-time RT-PCR primers and reaction conditions

From: Basal keratin expression in breast cancer by quantification of mRNA and by immunohistochemistry

Gene primers (5'-3')

Forward

Reverse

Annealing temperature ( ° C)

Detection temperature ( ° C)

PCR product size (base pairs)

Beta-2-microglobulin ( B2M )

TGAGTGCTGTCTCCATGTTTGA

TCTGCTCCCCACCTCTAAGTTG

50

81

88

H3 histone, family 3A ( H3F3A )

AGGACTTTAAAAGATCTGCGCTTCCAGAG

ACCAGATAGGCCTCACTTGCCTCCTGC

65

72

76

Ribosomal phosphoprotein ( RPLP0 )

ACGGATTACACCTTCCCACTTGCTAAAAGGTC

AGCCACAAAGGCAGATGGATCAGCCAAG

65

72

69

Ribosomal protein S17 ( RPS17 )

ACCCCAATGTCAAGGAGATCAAGGTCCTG

TCGGCAGCCAGCTCGTGAGTAATG

64

72

87

Estrogen receptor 1 ( ER )

ATCTCGGTTCCGCATGATGAATCTGC

TGCTGGACAGAAATGTGTACACTCCAGA

65

72

98

Keratin 5 (CK5)

ATCGCCACTTACCGCAAGCTGCTGGAGGG

AAACACTGCTTGTGACAACAGAG

65

72

102

Keratin 17 ( CK17 )

ATGTGAAGACGCGGCTGGAGCAGGA

ACCTGACGGGTGGTCACCGGTTC

65

72

109

Keratin 14 ( CK14 )

TTTGGCGGCTGGAGGAGGTCACA

ATCGCCACCTACCGCCGCCTG

65

72

109