Figure 3
Expression of mRNA encoding for VEGF, Flk-1 and MMP-9 in eutopic endometrium and endometriotic lesions as assayed by RT-PCR (A) and densitometry of bands (B). Lane 1, negative control (no cDNA); Lane 2, eutopic endometrium; Lane 3, lesions after 15 days; Lane 4, lesions after 30 days. The levels of VEGF, Flk-1 and MMP-9 mRNA transcripts in the endometriotic lesions were higher than in eutopic endometrium. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA was studied as constitutive housekeeping genes.