Figure 1

2D electrophoresis profiles of PcDNA3.1( IGFBP7 )-RKO-transfectants and PcDNA3.1-RKO transfectants. A. 2D electrophoresis profiles of silver staining proteins of PcDNA3.1(IGFBP7)-RKO transfectants (BP7-RKO) and PcDNA3.1-RKO transfectants (control). 0.75 milligrams of protein were loaded onto linear IPG strips (pH 5-8) and isoelectric focusing was performed at 35 kV-h. The second dimensional run was performed on 12.5% Tris-glycine-PAGE gels and the gels were stained with silver for image analysis. Protein spot discrepancies were arrowed and marked with number. B. Close-up image of differential expression of protein spots.