Downregulation of HSP60 protein expression in PcDNA3.1( IGFBP7 )-RKO transfectants. A: Whole cell lysates of the stable PcDNA3.1(IGFBP7)-RKO transfectants and PcDNA3.1-RKO transfectants were prepared, and equal amounts of protein (50 μg/lane) were loaded. HSP60 expression was assessed using a rabbit anti-HSP60 antibody. GAPDH is used as an internal loading control. Shown is representive of experiments performed on at least three different isolations. The amount of HSP60 protein expression in PcDNA3.1(IGFBP7)-RKO transfectants was lower than that of the control group. B: HSP60 concentration in cell supernatants was measured using the HSP60 ELISA kit according to the manufactures instructions. Experiments were performed in triplicates. Results represent the mean HSP60 concentration (ng/ml) ± SD,. *, p < 0:05 vs. control.