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Table 1 Description of different types of changes in gene expression after combined treatment with ATRA and inhibitors (CA or CX) in SK-N-BE(2) and SH-SY5Y cell lines

From: Enhancement of ATRA-induced differentiation of neuroblastoma cells with LOX/COX inhibitors: an expression profiling study

cluster

number of genes

type of change in gene expression

I. Treatment with ATRA and CA; SK-N-BE(2) cell line

I.A

7

strong increase especially after treatment with 10 ATRA/52 CA; marked increase noted also after treatment with 1 ATRA alone and all other combinations

I.B

14

marked increase especially after treatment with 1 ATRA/13 CA; the increase noted also after treatment with 1 ATRA alone

I.C

18

marked increase especially after treatment with 1 ATRA and also both combinations of ATRA with CA; 10 ATRA alone or in combinations with CA decreases gene expression

I.D

6

slight increase after treatment with 1 RA; marked decrease after treatment with 10 RA and all combinations

I.E

5

decrease after treatment with 10 ATRA in both combinations with CA

II. Treatment with ATRA and CA; SH-SY5Y cell line

II.A

12

strong increase especially after treatment with 10 ATRA/52 CA; marked increase noted also after treatment with 10 ATRA alone and all other combinations in concentration-dependent manner

II.B

58

marked increase especially after treatment with 1 ATRA in both combinations with CA and also after treatment with 10 ATRA/52 CA; application of ATRA alone showed no influence on gene expression

II.C

27

marked increase after treatment with 1 ATRA in both combinations; application of ATRA alone and 10 ATRA in both combinations showed no influence on gene expression

II.D

4

strong increase after treatment with 10 ATRA/52 CA; application of ATRA alone and all other combinations showed no or minimal influence on gene expression

III. Treatment with ATRA and CX; SK-N-BE(2) cell line

III.A

6

strong increase after treatment with 10 ATRA/10 CX and 1 ATRA/50 CX; slight increase after treatment with 1 ATRA/10 CX; application of ATRA alone showed no or minimal influence on gene expression

III.B

6

marked increase after treatment with ATRA in all combinations with CX; treatment with 1 ATRA alone showed the same effect on gene expression as observable in control cells

III.C

22

strong increase after treatment with ATRA in all combinations with CX; slight increase after treatment with 1 ATRA alone

III.D

4

marked increase after treatment with ATRA in all combinations with CX; decrease after treatment with ATRA alone

III.E

60

strong increase after treatment with 1 ATRA/10 CX; slight increase after treatment with 1 ATRA alone

IV. Treatment with ATRA and CX; SH-SY5Y cell line

IV.A

15

marked increase after treatment with 10 ATRA alone and also in both combinations with CX; application of 1 ATRA alone or in combinations with CX showed no or minimal influence on gene expression

IV.B

15

strong increase after treatment with 1 ATRA/10 CX; slight increase after treatment with 10 ATRA in both combinations with CX

IV.C

32

strong increase after treatment with 10 ATRA/50 CX

IV.D

4

marked increase after treatment with 1 ATRA/10 CX; marked decrease after treatment with all other combinations

  1. ATRA was applied in concentrations of 1 or 10 μM (1 ATRA, 10 ATRA); CA in concentrations of 13 and 52 μM (13 CA, 52 CA), and CX in concentrations of 10 and 50 μM (10 CX, 50 CX). All decriptions are related to the gene expression identified in control untreated cells.