Figure 1
From: Generation of functional CD8+ T Cells by human dendritic cells expressing glypican-3 epitopes
Expression of transgenes in monocyte-derived dendritic cells following transfection by electroporation of mRNA. a. Diagram of expression vector between SP6 transcription initiation site and SnaB1 restriction enzyme site. b. Denaturing agarose gel showing in vitro transcribed eGFP and GPC-3 mRNA. c. eGFP expression in monocyte-derived DC as determined by flow cytometry, 20 hours after mock transfection (filled area) or transfection with 20 μg eGFP mRNA (open area), when 64% of DC were positive for eGFP. d. GPC-3 expression in matured, monocyte-derived DC as determined by flow cytometry after staining with anti-GPC-3 antibody; left panel shows cell surface expression of GPC-3, 20 hours after mock transfection or transfection with 20 μg GPC-3 mRNA, staining with isotype control antibody is shown for comparison; right panel shows intracellular expression after DC were permeabilised by drop-wise addition of the cells to ice cold 70% ethanol, 20 hours after mock transfection (thick line) or transfection with 20 μg GPC-3 mRNA (pale line), staining with isotype control antibody is shown for comparison (filled area).