Figure 2

VEGF and MMP-2 mRNA levels in SW1990 and Capan-2 cells were detected by real time PCR. The extracted total RNA was reverse-transcribed into single-stranded cDNA, and real-time PCR was performed. Interleukin-6 (IL-6) markedly increased MMP-2 and VEGF mRNA expression in Capan-2 cells(P = 0.000, P = 0.000). AG490 significantly decreased MMP-2 and VEGF mRNA expression in SW1990 cells(P = 0.008, P = 0.000). β-actin was used as an endogenous control. * P < 0.01, versus Capan-2 cell group; #P < 0.01, versus SW1990 cell group.